Molecular Devices' microplate readers. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Plate reader instructions Computer. Microplate readers use a vertical light path so the distance of the. tecHnical SPecificationS Performance Specifications Measurement range: 400–750 nm 0–4. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. Microplate reader assays requiring infrared (IR) or red-shifted. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. 50 µL of. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. g. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 40266. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Pick the best speed, sensitivity, and price for your needs. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. microplate readers. Compare specifications and request a quote from leading suppliers. Instrument settings can be saved as a protocol file and used repeatedly for reading differentThe Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Outer wells are aligned for proper viewing with FLIPR. 300 µL of Detergent Compatible Bradford reagent was added to each well. for ValitaTiter Plus standards read on Molecular Devices readers. iD5. Compare this item. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. #3676). Brand: MOLECULAR DEVICES. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. ValitaTiter Plus 96-well (1 plate) VAL004. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Contents of wells were pipetted up and down several times to mix. maximum-illumination-channels. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. Learn more 0200-7008 6- to 1536-well plates. These robust readers have been placed in labs from. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The SpectraMax® ABS Plus Microplate Reader can run . Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Lucid Scientific. Wide range of applications The VersaMax reader covers. Vrla used the device to quantify the concentration of a small molecule produced by. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Published Dec 16, 2020 | Updated Dec 16, 2020. 0 500 1000 1500 2000 2500 3000 3500 time (sec. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. 96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-VolumeLearn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. 1 OD at 230 nm. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. ( a ) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Learn more. Also refer to this Multimode Reader Comparison Chart. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. This can increase throughput and efficiency, thus saving time and space in the lab. iD5. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. 4 Pages. SpectraMax® Microplate Reader Suite of Products Knowledge Base. The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. 2. AquaMax 4000 Microplate Washer (Molecular Devices Cat. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. 96-well: 0. Affordable multi-mode microplate reader with . adjust the reader. . The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. Features. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Visible absorbance -. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Paradigm: Delta mP. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Make sure to Data 96孔微孔板的数据对比. 3:40 . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Fluorescent DNA quantitation. Blank wells containing 50 µL culture medium only (n = 8) were also prepared. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. Description. Go to Product Details. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. < 0. Molecular Devices' microplate readers. 03 pM 384-well: 0. com. Instrument settings are listed in Table 1. 1. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. , 384 wells < 45 sec. 4. 96-well flat clear bottom white TC-treated microplates (Corning cat. 3 OD at 200 nm. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. book Page 9 Wednesday, July 7, 2010 2:09 PM. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. Read one sample or up to 384 in a. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. In addition, the iD5 reader measures TRF and FP and. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Endpoint, kinetic, spectrum. The system is operated through the industry-leading SoftMax® Pro. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). These robust readers have been placed in labs from Antarctica to. Wavelength Range 200 to 999 nm. 1536-well: 0. We would like to show you a description here but the site won’t allow us. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. reagent basin for ease of pipetting into. 48 W x 12. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. SmartReader 96: Designed for optimal ELISA Testing. the iD5 reader’s filter system. 5. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. The iMark. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. 1 FP • Fluorescence Polarization detection for fluorescein- or . a white 96-well half-area microplate (Corning cat. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. 0 96 Well Plate. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The screw remains inside the instrument. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. This reader provides visible. Supplier: Molecular Devices. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Page: 1/4. Average StDev (mP) 1. Contents of wells were pipetted up and down several times to mix. #T-0254) • Lysozyme (Sigma cat. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. 2 Pages. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan molecular devices Japan, osaka. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Taking into account the price differences between each seller and each country. LVF Monochromator™ : Excitation and Emission. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Reactions catalyzed by firefly and *Renilla luciferases. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. CloneMatrix. Timothy Bolus, MPM, MBA, Compliance Program Manager. 500 USD. EMax Plus Microplate Reader, Molecular Devices,96 Well Plate, LLC (CAT#: STEM-LE-0510-LC) Cytation™ 1 Cell Imaging Multi-Mode Reader, Wavelength Range: 200 to 700 nm, BioTek Instruments (CAT#: STEM-MR-0003-LKN) Perkin Elmer EnVision 2105-0020 Multilabel Microplate Reader, Perkin Elmer (CAT#: STEM-S-0723-LGZ)The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. Omnia® Compatible Microplate Reader Documentation Version No. #W50-100) SpectraMax® ABS Plus Microplate Reader. Retrieving. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. A. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 1 sec. Step 3: Click “Settings” and a screen will appear (Figure 1). 14: Average %CV. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Step 2: Launch SoftMax Pro Software. GxP regulated industry assessments of microplate readers. Four wash/rinse bottles are included and configurable for both 96- and 384. SpectraDrop High-Throughput Screening (HTS) Kit. We would like to show you a description here but the site won’t allow us. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 1 OD at 230 nm. Integrated stacker option. 14: Average %CV. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. analysis software. 100 fmol 384-well (75 µL) 0. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. All stand-alone instrument functions can be controlled using the software. Keywords: fluorescence polarization, trf, fret, igg. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. Bioz Stars score: 86/100, based on 1 PubMed citations. 020 fmol 0. 00 Add to cart; Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. In simplest terms, a plate reader measures and. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 96-well microplate at 200 µL/well. , Sunnyvale, CA 94089. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. style. the appropriate well of the microplate. System setup and. Wavelength Range. This page contains the. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. explore more. Technical specifications. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. In . buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. Filters: Excitation and emission slides for 4 filters each. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. The contents of the SpectraDrop Microplate kit depend on kit type. to each well. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Plate Formats: 6 to 384-well microplates. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. Microplate is validated by molecular devices for the FLIPR reader. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Industry standard, 96-well plates are. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Molecular Devices M5 Plate Reader Plate Calibration Form . Cross-talk < 0. 5°C at 37°C. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. section, select plate section titled. 1 pM 1536-well: 0. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. fluorescence microplate reader from Molecular Devices. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. The plate reader optics are tunable for. 1 pM. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. A control well was set up to verify. Monochromator, tunable in 1-nm increments. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The system provides automation for up to 50 microplates for easy walkaway automation. #12-639) White 96- and 384-well microplates (Greiner cat. Products : General, FilterMax F5 reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Optical System: High energy xenon flash lamp. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Microplate Readers. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Microplate Readers. 0. #N207) LAL Reagent Water (Lonza cat. 747. We would like to show you a description here but the site won’t allow us. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. Fluorescence Microplate Reader Comparison Chart. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. measurements in compatible Molecular Devices® microplate readers. Standard SBS 96 and 384 well plates (128. Condition: Used - Good. 125 pM. Microplate Formats: 6, 12, 24, 48, 96, 384. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. tools to expand the boundaries of their research. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. 2 OD at 200 nm. Endpoint, kinetic, spectrum and area-well scanning Click here. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. A microplate reader as . An industry-renowned instrument for monitoring GPCRs and ion channels. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. BSA concentration (µg/mL) Average OD. 2 mm x 86 mm) Height: between 13. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. automation for any lab. Microplates . OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. Page 154: Ordering Parts And Accessories. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. 0 500 1000 1500 2000 2500 3000 3500 time (sec. #PLUS 384) Methods. It features a modular design that allows users to add or change detection cartridges according to their needs. With optimized reagents, option to operate the. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Related Products: Low Binding Plates. 585The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. to each well. 3% in white 96- and. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. 7. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. 030 fmol Fluorescence Polarization (FP). ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. ©2017 Molecular Devices, LLC . Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 5% at 1. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Fluorescence Microplate Reader Comparison Chart. Description. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. 384 Awareness Technology ChroMate BioTek Synergy Mx. The Molecular Devices Versamax Microplate Reader INCLUDED:. While "plate reader" usually refers to the devices described. Expt#3 . The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). Keywords: fluorescence polarization, trf, fret, igg. Insert the filled microplate into the drawer, matching well A1 with position A1. > Molecular Devices LLC > microplate reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 1/17 1907B Printed in USA . Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. Compact and tunable absorbance microplate readers DATA SHEET. Detergent Compatible Bradford assay. 63. For more sample throughput, the system can be. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. 003 fmol 0. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Other technologies such as imaging. Description. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Simple, economical . Microplate Reader (Molecular Devices cat. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. #M5e) Methods Microplate reader compatibility test. microplates or in a cuvette at a selected.